The aim of the present work is to develop simple accurate, precise and cost effective UV-Vis spectrophotometric method for the estimation of pamabrom in bulk and tablet dosage form determination of percent degradation by using different stress condition. Pamabrom shows the maximum absorbance (λmax) at 279 nm in distilled water and was utilized for its determination. Drug was found to follow the Beer-Lambert’s law in the concentration range of 2-20 μg/ml. The suggested method is validated by using ICH validation parameters like accuracy, precision, linearity, LOD and LOQ respectively. Standard deviation and RSD for intra-day and inter-day precision studies was found to be less than ± 2. The limit of detection and limit of quantification was found to be 0.239 μg/ml & 0.725 μg/ml. The proposed method was successfully applied to the determination of pamabrom in pharmaceutical formulations without any interference from excipients. Results of the analysis were validated statistically and found to be satisfactory. Pamabrom was subjected to stress degradation under different conditions recommended by ICH. The samples generated were used for degradation studies using the developed method.
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